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Cambistat

Improve Water Relations

Stomatal Aperture

Trees treated with Cambistat may have elevated levels of the hormone abscisic acid that results from the interruption of the production of gibberellic acid in the isoprenoid pathway. Abscisic acid plays a key role in the aperture of the stomata. It is naturally produced in root tips in response to dry soils. From the roots it is transported through the xylem into the canopy and eventually the leaves. In the leaves, abscisic acid signals the guard cells of the stomata to close. By elevating levels of this hormone with Cambistat stomatal aperture is more conservative and water status is maintained. Research with growth regulators demonstrates this effect, as seen in the chart on the right.
In this study by Dr. Bill Chaney at Purdue University, small trees were exposed to drought in a greenhouse. Trees treated with the growth regulator (heavy line) maintained higher leaf water content for a longer period of time than untreated controls (dashed line).
   
 
  This photo shows the method for measuring leaf relative water content. Fresh leaf tissue samples are weighed, fully re-hydrated in test tubes as shown here, and then re-weighed. Water status is indicated by comparison of the fresh weight with the fully hydrated weight.

All photos courtesy of Dr.Bill Chaney – Purdue University

Thicker Leaves with More Trichomes

When trees are treated with Cambistat there is an increase in Chlorophyll and Abscisic acid, which can cause the tree to produce thicker, darker green leaves. Leaves of some species also appear to produce a significantly higher number of trichomes (hairs), which may provide a physical barrier to water loss.
       
untreated treated  untreated treated 
These electron microscope photographs show the leaves of treated and untreated trees. Notice how much thicker the treated tree leaf is. These electron microscope photographs show the undersides of leaves of treated and untreated trees. Notice how many more trichomes (hairs) have grown on leaves of treated trees. This response may help explain decreases in water loss and increased defenses of leaves on treated trees.

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